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addgene pgex shp 1 nc sh2  (Addgene inc)


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    Addgene inc addgene pgex shp 1 nc sh2
    Addgene Pgex Shp 1 Nc Sh2, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/addgene+pgex+shp+1+nc+sh2/pGEX+SHP-1(NC)-SH2+(Plasmid+%2346496)/pmc08477956-54-8-8
    Average 92 stars, based on 2 article reviews
    addgene pgex shp 1 nc sh2 - by Bioz Stars, 2026-09
    92/100 stars

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    Plasmid Preparation:

    Article Title: Antibody:CD47 ratio regulates macrophage phagocytosis through competitive receptor phosphorylation
    Article Snippet: Second generation Lenti virus packaging plasmids pMD2.G and p8.91 , CloneTech , N/A. .. pHR-SHP1-SH2-GFP , this paper , SHP1 SH2 from Addgene (pGEX-SHP-1(NC)-SH2, plasmid #46496). ..

    Article Title: Antibody:CD47 ratio regulates macrophage phagocytosis through competitive receptor phosphorylation
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-biotin antibody (clone BK1/39, AlexaFluor647) Santa Cruz Biotechnology RRID: AB_2892070 Anti-human HER2 antibody (Trastuzumab epitope target, mouse IgG1) Aduro Biotech N/A Anti-SIRPa antibody (clone 18a, D265A Fc mutant) Aduro Biotech N/A Anti-phosphotyrosine antibody (P-Y-1000 MultiMab) Cell Signaling Technology RRID: AB_2687925 Anti-rabbit secondary antibody (AlexaFluor546 anti-rabbit goat IgG) Thermo Scientific RRID:AB_2534077 Anti-CD45 antibody (clone 30-F11, Brilliant Violet 421) Biolegend RRID: AB_10899570 Mouse Fc Block BD Biosciences Cat# 553142 Anti-CD47 antibody (clone MIAP 301) Aduro Biotech N/A Anti-SIRPa antibody (clone p84) Aduro Biotech N/A Bacterial and virus strains Rosetta2 (DE3) pLysS UC Berkeley MacroLab N/A XL1-Blue UC Berkeley MacroLab N/A Chemicals, peptides, and recombinant proteins TransIT-293 Mirus Bio Cat# MIR 2700 POPC (1-palmitoyl-2-oleoyl-sn-glycero-3phosphocholine) Avanti Polar Lipids Cat# 850457 Recombinant mouse CD47 (His tagged) SinoBiological Cat# 57231-M08H Alexa Fluor 488 NHS Ester Thermo Fisher Cat# A20000 CellTracker Deep Red Thermo Fisher Cat# C34565 Alexa Fluor 647 NHS Ester Thermo Fisher Cat# A37573 DGS-Ni-NTA (1,2-dioleoyl-sn-glycero-3[(N-(5-amino-1-carboxypentyl) iminodiacetic acid)succinyl], with nickel salt) Avanti Polar Lipids Cat# 709404 NSC-87877, SHP1/2 Inhibtor Sigma-Aldrich Cat# 56932-43-5 Biotinyl Cap PE (1,2-dioleoyl-sn-glycero-3phosphoethanolamine-N-(cap biotinyl)) Avanti Polar Lipids Cat# 870273 LISS-Rhodamine (1,2-dioleoyl-sn-glycero3-phosphoethanolamine-N-(lissamine rhodamine B sulfonyl)) Avanti Polar Lipids Cat# 810150 CMFDA (CellTracker Green) Thermo Fisher Cat# C2925 Hoechst 33342 Thermo Fisher Cat# H3570 PP2, Src family kinase inhibitor Sigma Aldrich Cat# 529573 Experimental models: Cell lines MC38 mouse colon carcinoma cell line Aduro Biotech N/A RAW 264.7 mouse macrophage-like cell line UC Berkeley Cell Culture Facility https://bds.berkeley.edu/facilities/ cell-culture Bone marrow derived macrophages, C57BL/6J background UC Berkeley Portnoy Lab N/A (Continued on next page) Cell Reports 36, 109587, August 24, 2021 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Recombinant DNA pHR-SHP1-SH2-mEOS this paper SHP1 SH2 from Addgene (pGEX-SHP- 1(NC)-SH2, plasmid #46496); mEOS pHR-Syn18-F1L-TM-FcgRIIA-ITAM-GFP this paper Syn18: SIAATLENDLARLENENAR LEKDIANLERDLAKLEREEAYF; F1L as in Bakalar et al. (2018); Transmembrane domain of mouse SIRPa (Uniprot #P97797, amino acid 374-394); ITAM of FcgRIIA (Uniprot #P12318, amino acid 240-317); EGFP pHR-Syn18-F1L-TM-CD3z-ITAM-GFP this paper As above except ITAM of CD3z (Uniprot #24161, amino acid 52-164) pHR-Syn18-F1L-TM-FcERIgamma-ITAMGFP this paper As above except ITAM of FcεRI g-chain (Uniprot #P20491, amino acid 45-86) pHR-Syn18-F1L-TM-FcgRIIB-ITIM-GFP this paper As above except ITIM of FcgRIIB (Uniprot #P08101, amino acid 232-330) pHR-Syn18-F1L-TM-SIRPa-ITIM-GFP this paper As above except ITIM of SIRPa (Uniprot #P97797, amino acid 395-513) pHR-Syn18-F1L-TM-CD22-ITIM-GFP this paper As above except ITIM of CD22 (Uniprot #P35329, amino acid 722-862) pHR-Syn18-F1L-TM-PD1-ITIM-GFP this paper As above except ITIM of PD-1 (Uniprot #Q02242, amino acid 191-288) pHR-Syn18-F1L-TM-GFP this paper As above except no ITAM or ITIM pet28-Syn17-F3L-H10-KCK-H6 this paper Syn17: NEKEELKSKKAELRNRI EQLKQKREQLKQKIANLRKEIEAYK; F3L as in Bakalar et al. (2018) pet28-Syn17-F5L-H10-KCK-H6 this paper Syn17: NEKEELKSKKAELRNR IEQLKQKREQLKQKIANLRKEIEAYK; F5L as in Bakalar et al. (2018) pHR-Syk-SH2-mCh Bakalar et al., 2018 N/A Second generation Lenti virus packaging plasmids pMD2.G and p8.91 CloneTech N/A pHR-SHP1-SH2-GFP this paper SHP1 SH2 from Addgene (pGEX-SHP-1(NC)-SH2, plasmid #46496) pHR-Syk-SH2-mEOS this paper Syk SH2 from Uniprot ID P48025; mEOS Software and algorithms Cell Profiler Broad Institute RRID: SCR_007358; https://cellprofiler.org/ MATLAB Mathworks RRID: SCR_001622; https://www. mathworks.com/products/matlab.html Python Python RRID: SCR_008394; https://www.python. org/ Fiji Image Analysis Fiji RRID:SCR_002285; https://imagej.net/ software/fiji/ Other 8-well glass bottom dishes Cellvis Cat# C8-1 Calibrated fluorescent beads, Quantum MESF Bangs Laboratories, Inc. Cat# 488, 647 4 mm non-functionalized silica bead Bangs Laboratories, Inc. Cat# SS05002 Article ll OPEN ACCESS ..

    Article Title: Antibody:CD47 ratio regulates macrophage phagocytosis through competitive receptor phosphorylation
    Article Snippet: .. pHR-SHP1-SH2-mEOS , this paper , SHP1 SH2 from Addgene (pGEX-SHP-1(NC)-SH2, plasmid #46496); mEOS. ..

    Recombinant:

    Article Title: Antibody:CD47 ratio regulates macrophage phagocytosis through competitive receptor phosphorylation
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-biotin antibody (clone BK1/39, AlexaFluor647) Santa Cruz Biotechnology RRID: AB_2892070 Anti-human HER2 antibody (Trastuzumab epitope target, mouse IgG1) Aduro Biotech N/A Anti-SIRPa antibody (clone 18a, D265A Fc mutant) Aduro Biotech N/A Anti-phosphotyrosine antibody (P-Y-1000 MultiMab) Cell Signaling Technology RRID: AB_2687925 Anti-rabbit secondary antibody (AlexaFluor546 anti-rabbit goat IgG) Thermo Scientific RRID:AB_2534077 Anti-CD45 antibody (clone 30-F11, Brilliant Violet 421) Biolegend RRID: AB_10899570 Mouse Fc Block BD Biosciences Cat# 553142 Anti-CD47 antibody (clone MIAP 301) Aduro Biotech N/A Anti-SIRPa antibody (clone p84) Aduro Biotech N/A Bacterial and virus strains Rosetta2 (DE3) pLysS UC Berkeley MacroLab N/A XL1-Blue UC Berkeley MacroLab N/A Chemicals, peptides, and recombinant proteins TransIT-293 Mirus Bio Cat# MIR 2700 POPC (1-palmitoyl-2-oleoyl-sn-glycero-3phosphocholine) Avanti Polar Lipids Cat# 850457 Recombinant mouse CD47 (His tagged) SinoBiological Cat# 57231-M08H Alexa Fluor 488 NHS Ester Thermo Fisher Cat# A20000 CellTracker Deep Red Thermo Fisher Cat# C34565 Alexa Fluor 647 NHS Ester Thermo Fisher Cat# A37573 DGS-Ni-NTA (1,2-dioleoyl-sn-glycero-3[(N-(5-amino-1-carboxypentyl) iminodiacetic acid)succinyl], with nickel salt) Avanti Polar Lipids Cat# 709404 NSC-87877, SHP1/2 Inhibtor Sigma-Aldrich Cat# 56932-43-5 Biotinyl Cap PE (1,2-dioleoyl-sn-glycero-3phosphoethanolamine-N-(cap biotinyl)) Avanti Polar Lipids Cat# 870273 LISS-Rhodamine (1,2-dioleoyl-sn-glycero3-phosphoethanolamine-N-(lissamine rhodamine B sulfonyl)) Avanti Polar Lipids Cat# 810150 CMFDA (CellTracker Green) Thermo Fisher Cat# C2925 Hoechst 33342 Thermo Fisher Cat# H3570 PP2, Src family kinase inhibitor Sigma Aldrich Cat# 529573 Experimental models: Cell lines MC38 mouse colon carcinoma cell line Aduro Biotech N/A RAW 264.7 mouse macrophage-like cell line UC Berkeley Cell Culture Facility https://bds.berkeley.edu/facilities/ cell-culture Bone marrow derived macrophages, C57BL/6J background UC Berkeley Portnoy Lab N/A (Continued on next page) Cell Reports 36, 109587, August 24, 2021 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Recombinant DNA pHR-SHP1-SH2-mEOS this paper SHP1 SH2 from Addgene (pGEX-SHP- 1(NC)-SH2, plasmid #46496); mEOS pHR-Syn18-F1L-TM-FcgRIIA-ITAM-GFP this paper Syn18: SIAATLENDLARLENENAR LEKDIANLERDLAKLEREEAYF; F1L as in Bakalar et al. (2018); Transmembrane domain of mouse SIRPa (Uniprot #P97797, amino acid 374-394); ITAM of FcgRIIA (Uniprot #P12318, amino acid 240-317); EGFP pHR-Syn18-F1L-TM-CD3z-ITAM-GFP this paper As above except ITAM of CD3z (Uniprot #24161, amino acid 52-164) pHR-Syn18-F1L-TM-FcERIgamma-ITAMGFP this paper As above except ITAM of FcεRI g-chain (Uniprot #P20491, amino acid 45-86) pHR-Syn18-F1L-TM-FcgRIIB-ITIM-GFP this paper As above except ITIM of FcgRIIB (Uniprot #P08101, amino acid 232-330) pHR-Syn18-F1L-TM-SIRPa-ITIM-GFP this paper As above except ITIM of SIRPa (Uniprot #P97797, amino acid 395-513) pHR-Syn18-F1L-TM-CD22-ITIM-GFP this paper As above except ITIM of CD22 (Uniprot #P35329, amino acid 722-862) pHR-Syn18-F1L-TM-PD1-ITIM-GFP this paper As above except ITIM of PD-1 (Uniprot #Q02242, amino acid 191-288) pHR-Syn18-F1L-TM-GFP this paper As above except no ITAM or ITIM pet28-Syn17-F3L-H10-KCK-H6 this paper Syn17: NEKEELKSKKAELRNRI EQLKQKREQLKQKIANLRKEIEAYK; F3L as in Bakalar et al. (2018) pet28-Syn17-F5L-H10-KCK-H6 this paper Syn17: NEKEELKSKKAELRNR IEQLKQKREQLKQKIANLRKEIEAYK; F5L as in Bakalar et al. (2018) pHR-Syk-SH2-mCh Bakalar et al., 2018 N/A Second generation Lenti virus packaging plasmids pMD2.G and p8.91 CloneTech N/A pHR-SHP1-SH2-GFP this paper SHP1 SH2 from Addgene (pGEX-SHP-1(NC)-SH2, plasmid #46496) pHR-Syk-SH2-mEOS this paper Syk SH2 from Uniprot ID P48025; mEOS Software and algorithms Cell Profiler Broad Institute RRID: SCR_007358; https://cellprofiler.org/ MATLAB Mathworks RRID: SCR_001622; https://www. mathworks.com/products/matlab.html Python Python RRID: SCR_008394; https://www.python. org/ Fiji Image Analysis Fiji RRID:SCR_002285; https://imagej.net/ software/fiji/ Other 8-well glass bottom dishes Cellvis Cat# C8-1 Calibrated fluorescent beads, Quantum MESF Bangs Laboratories, Inc. Cat# 488, 647 4 mm non-functionalized silica bead Bangs Laboratories, Inc. Cat# SS05002 Article ll OPEN ACCESS ..

    Virus:

    Article Title: Antibody:CD47 ratio regulates macrophage phagocytosis through competitive receptor phosphorylation
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-biotin antibody (clone BK1/39, AlexaFluor647) Santa Cruz Biotechnology RRID: AB_2892070 Anti-human HER2 antibody (Trastuzumab epitope target, mouse IgG1) Aduro Biotech N/A Anti-SIRPa antibody (clone 18a, D265A Fc mutant) Aduro Biotech N/A Anti-phosphotyrosine antibody (P-Y-1000 MultiMab) Cell Signaling Technology RRID: AB_2687925 Anti-rabbit secondary antibody (AlexaFluor546 anti-rabbit goat IgG) Thermo Scientific RRID:AB_2534077 Anti-CD45 antibody (clone 30-F11, Brilliant Violet 421) Biolegend RRID: AB_10899570 Mouse Fc Block BD Biosciences Cat# 553142 Anti-CD47 antibody (clone MIAP 301) Aduro Biotech N/A Anti-SIRPa antibody (clone p84) Aduro Biotech N/A Bacterial and virus strains Rosetta2 (DE3) pLysS UC Berkeley MacroLab N/A XL1-Blue UC Berkeley MacroLab N/A Chemicals, peptides, and recombinant proteins TransIT-293 Mirus Bio Cat# MIR 2700 POPC (1-palmitoyl-2-oleoyl-sn-glycero-3phosphocholine) Avanti Polar Lipids Cat# 850457 Recombinant mouse CD47 (His tagged) SinoBiological Cat# 57231-M08H Alexa Fluor 488 NHS Ester Thermo Fisher Cat# A20000 CellTracker Deep Red Thermo Fisher Cat# C34565 Alexa Fluor 647 NHS Ester Thermo Fisher Cat# A37573 DGS-Ni-NTA (1,2-dioleoyl-sn-glycero-3[(N-(5-amino-1-carboxypentyl) iminodiacetic acid)succinyl], with nickel salt) Avanti Polar Lipids Cat# 709404 NSC-87877, SHP1/2 Inhibtor Sigma-Aldrich Cat# 56932-43-5 Biotinyl Cap PE (1,2-dioleoyl-sn-glycero-3phosphoethanolamine-N-(cap biotinyl)) Avanti Polar Lipids Cat# 870273 LISS-Rhodamine (1,2-dioleoyl-sn-glycero3-phosphoethanolamine-N-(lissamine rhodamine B sulfonyl)) Avanti Polar Lipids Cat# 810150 CMFDA (CellTracker Green) Thermo Fisher Cat# C2925 Hoechst 33342 Thermo Fisher Cat# H3570 PP2, Src family kinase inhibitor Sigma Aldrich Cat# 529573 Experimental models: Cell lines MC38 mouse colon carcinoma cell line Aduro Biotech N/A RAW 264.7 mouse macrophage-like cell line UC Berkeley Cell Culture Facility https://bds.berkeley.edu/facilities/ cell-culture Bone marrow derived macrophages, C57BL/6J background UC Berkeley Portnoy Lab N/A (Continued on next page) Cell Reports 36, 109587, August 24, 2021 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Recombinant DNA pHR-SHP1-SH2-mEOS this paper SHP1 SH2 from Addgene (pGEX-SHP- 1(NC)-SH2, plasmid #46496); mEOS pHR-Syn18-F1L-TM-FcgRIIA-ITAM-GFP this paper Syn18: SIAATLENDLARLENENAR LEKDIANLERDLAKLEREEAYF; F1L as in Bakalar et al. (2018); Transmembrane domain of mouse SIRPa (Uniprot #P97797, amino acid 374-394); ITAM of FcgRIIA (Uniprot #P12318, amino acid 240-317); EGFP pHR-Syn18-F1L-TM-CD3z-ITAM-GFP this paper As above except ITAM of CD3z (Uniprot #24161, amino acid 52-164) pHR-Syn18-F1L-TM-FcERIgamma-ITAMGFP this paper As above except ITAM of FcεRI g-chain (Uniprot #P20491, amino acid 45-86) pHR-Syn18-F1L-TM-FcgRIIB-ITIM-GFP this paper As above except ITIM of FcgRIIB (Uniprot #P08101, amino acid 232-330) pHR-Syn18-F1L-TM-SIRPa-ITIM-GFP this paper As above except ITIM of SIRPa (Uniprot #P97797, amino acid 395-513) pHR-Syn18-F1L-TM-CD22-ITIM-GFP this paper As above except ITIM of CD22 (Uniprot #P35329, amino acid 722-862) pHR-Syn18-F1L-TM-PD1-ITIM-GFP this paper As above except ITIM of PD-1 (Uniprot #Q02242, amino acid 191-288) pHR-Syn18-F1L-TM-GFP this paper As above except no ITAM or ITIM pet28-Syn17-F3L-H10-KCK-H6 this paper Syn17: NEKEELKSKKAELRNRI EQLKQKREQLKQKIANLRKEIEAYK; F3L as in Bakalar et al. (2018) pet28-Syn17-F5L-H10-KCK-H6 this paper Syn17: NEKEELKSKKAELRNR IEQLKQKREQLKQKIANLRKEIEAYK; F5L as in Bakalar et al. (2018) pHR-Syk-SH2-mCh Bakalar et al., 2018 N/A Second generation Lenti virus packaging plasmids pMD2.G and p8.91 CloneTech N/A pHR-SHP1-SH2-GFP this paper SHP1 SH2 from Addgene (pGEX-SHP-1(NC)-SH2, plasmid #46496) pHR-Syk-SH2-mEOS this paper Syk SH2 from Uniprot ID P48025; mEOS Software and algorithms Cell Profiler Broad Institute RRID: SCR_007358; https://cellprofiler.org/ MATLAB Mathworks RRID: SCR_001622; https://www. mathworks.com/products/matlab.html Python Python RRID: SCR_008394; https://www.python. org/ Fiji Image Analysis Fiji RRID:SCR_002285; https://imagej.net/ software/fiji/ Other 8-well glass bottom dishes Cellvis Cat# C8-1 Calibrated fluorescent beads, Quantum MESF Bangs Laboratories, Inc. Cat# 488, 647 4 mm non-functionalized silica bead Bangs Laboratories, Inc. Cat# SS05002 Article ll OPEN ACCESS ..

    Software:

    Article Title: Antibody:CD47 ratio regulates macrophage phagocytosis through competitive receptor phosphorylation
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-biotin antibody (clone BK1/39, AlexaFluor647) Santa Cruz Biotechnology RRID: AB_2892070 Anti-human HER2 antibody (Trastuzumab epitope target, mouse IgG1) Aduro Biotech N/A Anti-SIRPa antibody (clone 18a, D265A Fc mutant) Aduro Biotech N/A Anti-phosphotyrosine antibody (P-Y-1000 MultiMab) Cell Signaling Technology RRID: AB_2687925 Anti-rabbit secondary antibody (AlexaFluor546 anti-rabbit goat IgG) Thermo Scientific RRID:AB_2534077 Anti-CD45 antibody (clone 30-F11, Brilliant Violet 421) Biolegend RRID: AB_10899570 Mouse Fc Block BD Biosciences Cat# 553142 Anti-CD47 antibody (clone MIAP 301) Aduro Biotech N/A Anti-SIRPa antibody (clone p84) Aduro Biotech N/A Bacterial and virus strains Rosetta2 (DE3) pLysS UC Berkeley MacroLab N/A XL1-Blue UC Berkeley MacroLab N/A Chemicals, peptides, and recombinant proteins TransIT-293 Mirus Bio Cat# MIR 2700 POPC (1-palmitoyl-2-oleoyl-sn-glycero-3phosphocholine) Avanti Polar Lipids Cat# 850457 Recombinant mouse CD47 (His tagged) SinoBiological Cat# 57231-M08H Alexa Fluor 488 NHS Ester Thermo Fisher Cat# A20000 CellTracker Deep Red Thermo Fisher Cat# C34565 Alexa Fluor 647 NHS Ester Thermo Fisher Cat# A37573 DGS-Ni-NTA (1,2-dioleoyl-sn-glycero-3[(N-(5-amino-1-carboxypentyl) iminodiacetic acid)succinyl], with nickel salt) Avanti Polar Lipids Cat# 709404 NSC-87877, SHP1/2 Inhibtor Sigma-Aldrich Cat# 56932-43-5 Biotinyl Cap PE (1,2-dioleoyl-sn-glycero-3phosphoethanolamine-N-(cap biotinyl)) Avanti Polar Lipids Cat# 870273 LISS-Rhodamine (1,2-dioleoyl-sn-glycero3-phosphoethanolamine-N-(lissamine rhodamine B sulfonyl)) Avanti Polar Lipids Cat# 810150 CMFDA (CellTracker Green) Thermo Fisher Cat# C2925 Hoechst 33342 Thermo Fisher Cat# H3570 PP2, Src family kinase inhibitor Sigma Aldrich Cat# 529573 Experimental models: Cell lines MC38 mouse colon carcinoma cell line Aduro Biotech N/A RAW 264.7 mouse macrophage-like cell line UC Berkeley Cell Culture Facility https://bds.berkeley.edu/facilities/ cell-culture Bone marrow derived macrophages, C57BL/6J background UC Berkeley Portnoy Lab N/A (Continued on next page) Cell Reports 36, 109587, August 24, 2021 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Recombinant DNA pHR-SHP1-SH2-mEOS this paper SHP1 SH2 from Addgene (pGEX-SHP- 1(NC)-SH2, plasmid #46496); mEOS pHR-Syn18-F1L-TM-FcgRIIA-ITAM-GFP this paper Syn18: SIAATLENDLARLENENAR LEKDIANLERDLAKLEREEAYF; F1L as in Bakalar et al. (2018); Transmembrane domain of mouse SIRPa (Uniprot #P97797, amino acid 374-394); ITAM of FcgRIIA (Uniprot #P12318, amino acid 240-317); EGFP pHR-Syn18-F1L-TM-CD3z-ITAM-GFP this paper As above except ITAM of CD3z (Uniprot #24161, amino acid 52-164) pHR-Syn18-F1L-TM-FcERIgamma-ITAMGFP this paper As above except ITAM of FcεRI g-chain (Uniprot #P20491, amino acid 45-86) pHR-Syn18-F1L-TM-FcgRIIB-ITIM-GFP this paper As above except ITIM of FcgRIIB (Uniprot #P08101, amino acid 232-330) pHR-Syn18-F1L-TM-SIRPa-ITIM-GFP this paper As above except ITIM of SIRPa (Uniprot #P97797, amino acid 395-513) pHR-Syn18-F1L-TM-CD22-ITIM-GFP this paper As above except ITIM of CD22 (Uniprot #P35329, amino acid 722-862) pHR-Syn18-F1L-TM-PD1-ITIM-GFP this paper As above except ITIM of PD-1 (Uniprot #Q02242, amino acid 191-288) pHR-Syn18-F1L-TM-GFP this paper As above except no ITAM or ITIM pet28-Syn17-F3L-H10-KCK-H6 this paper Syn17: NEKEELKSKKAELRNRI EQLKQKREQLKQKIANLRKEIEAYK; F3L as in Bakalar et al. (2018) pet28-Syn17-F5L-H10-KCK-H6 this paper Syn17: NEKEELKSKKAELRNR IEQLKQKREQLKQKIANLRKEIEAYK; F5L as in Bakalar et al. (2018) pHR-Syk-SH2-mCh Bakalar et al., 2018 N/A Second generation Lenti virus packaging plasmids pMD2.G and p8.91 CloneTech N/A pHR-SHP1-SH2-GFP this paper SHP1 SH2 from Addgene (pGEX-SHP-1(NC)-SH2, plasmid #46496) pHR-Syk-SH2-mEOS this paper Syk SH2 from Uniprot ID P48025; mEOS Software and algorithms Cell Profiler Broad Institute RRID: SCR_007358; https://cellprofiler.org/ MATLAB Mathworks RRID: SCR_001622; https://www. mathworks.com/products/matlab.html Python Python RRID: SCR_008394; https://www.python. org/ Fiji Image Analysis Fiji RRID:SCR_002285; https://imagej.net/ software/fiji/ Other 8-well glass bottom dishes Cellvis Cat# C8-1 Calibrated fluorescent beads, Quantum MESF Bangs Laboratories, Inc. Cat# 488, 647 4 mm non-functionalized silica bead Bangs Laboratories, Inc. Cat# SS05002 Article ll OPEN ACCESS ..



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    addgene pgex shp 1 nc sh2  (Addgene inc)
    92
    Addgene inc addgene pgex shp 1 nc sh2
    Addgene Pgex Shp 1 Nc Sh2, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/addgene+pgex+shp+1+nc+sh2/pGEX+SHP-1(NC)-SH2+(Plasmid+%2346496)/pmc08477956-54-8-8
    Average 92 stars, based on 1 article reviews
    addgene pgex shp 1 nc sh2 - by Bioz Stars, 2026-09
    92/100 stars
    		  Buy from Supplier

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